Translational features of human alpha 2b interferon production in Escherichia coli.
نویسندگان
چکیده
The yield of human alpha 2b interferon in Escherichia coli was optimized by replacement of low-usage arginine codons located in the mRNA 5' end. The differences observed among the various gene variants suggest that codon usage, Shine-Dalgarno-like sequences, and mRNA secondary structure contribute to the performance of E. coli translation machinery.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 70 8 شماره
صفحات -
تاریخ انتشار 2004